![]() The 97,565 PUTs that were at least 100 bp in length were functionally annotated by a similarity search against public databases and assigned with Gene Ontology (GO) terms. These EST reads were clustered and assembled into 114,124 putative unique transcripts (PUTs) with an average length of 536 bp. By using the 454 pyrosequencing technology, a total of 1,571,764 high-quality reads were generated, among which 733,128 were from vegetative buds and 775,636 were from female buds. In order to gain insight into the molecular mechanisms behind the formation of reproductive buds and the transition from vegetative phase to reproductive phase in Metasequoia, we performed sequencing of expressed sequence tags from Metasequoia vegetative buds and female buds. Despite its paleontological significance and economical value as a rare relict species, genomic resources of Metasequoia are very limited. The "living fossil" Metasequoia glyptostroboides Hu et Cheng, commonly known as dawn redwood or Chinese redwood, is the only living species in the genus and is valued for its essential oil and crude extracts that have great potential for anti-fungal activity. Zhao, Ying Thammannagowda, Shivegowda Staton, Margaret Tang, Sha Xia, Xinli Yin, Weilun Liang, Haiying It also takes sustainable design to a new level: Not only is the structure built using sustainable materials and construction methods, but it solely exists to foster a sustainable relationship between humans and the environment.An EST dataset for Metasequoia glyptostroboides buds: the first EST resource for molecular genomics studies in Metasequoia. ![]() The viewing structure has reinvigorated the arboretum visitors’ interest and interaction with the Metasequoias. In the basket, visitors may sit on wooden benches while they contemplate their surrounds and listen to an interpretive audio piece that tells the story of Metasequoia trees. As visitors ascend the stairs to the basket, bolted connections in the structure’s steel frame allow it to flex with their movements, similar to the sway of tree branches subjected to load and wind. To minimize its impact with the trees’ complex root system, the structure has only three support legs, creating a lightness that makes the basket, a brightly colored, open-air observation deck, appear suspended in the tree canopy. Respectful of its surrounding landscape, the structure is virtually indiscernible except for the “branches” from which visitors view the trees. Working with the artists, KSS Architects translated their visions into a viewing structure that gives visitors the ability to enter and experience the tree canopies as easily as birds. Two artists, commissioned by the arboretum to build a new on-site exhibition, became enchanted with the grove, and wanted to create an installation that would re-engage visitors to the Metasequoias by sending visitors up into the trees. Accompanying their growing stature, unfortunately, was their growing isolation from the children and adults enjoying the open expanses of land below them. Revered for their rapid growth and beautiful straight trunks, the Dawn redwoods had surpassed 100 feet since their introduction to the arboretum in the 1950s. For more than 50 years, a majestic grove of Metasequoia trees has risen high above the verdant landscape of the Morrisville Arboretum.
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